Figure 2: Construction and application of the proposed comprehensive 2D K562/CMC system Scheme of the 2D K562/CMC system. | Scientific Reports

Figure 2: Construction and application of the proposed comprehensive 2D K562/CMC system Scheme of the 2D K562/CMC system.

From: Characterization of anti-leukemia components from Indigo naturalis using comprehensive two-dimensional K562/cell membrane chromatography and in silico target identification

Figure 2

(a) K562/CMC column was equilibrated, and the 1st fraction was collected in pre-column 1. (b) The 1st fraction was analyzed by a C18 column coupled with TOFMS while the 2nd fraction was collected in pre-column 2, then into the C18 column and TOFMS for analysis, alternately. (c) 3D plot of mixed standards obtained by 2D K562/CMC system. Dexamethasone was used as negative control, and showed no retention behavior on K562/CMC. Imatinib was used as positive control, and showed a significant retention characteristic on the K562/CMC. (d) 3D plot of Qingdai extracts obtained by 2D K562/CMC system. Three significant retention constituents were identified as indirubin, tryptanthrin and isorhamnetin, and one minor retention constituent was identified as indigo. (e) Chemical structures of potential bioactive components screened in Qingdai extracts.

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