Table 1 Primers designed for the amplifications of rpb1 and tef1α.

From: Multilocus phylogenetic analyses reveal unexpected abundant diversity and significant disjunct distribution pattern of the Hedgehog Mushrooms (Hydnum L.)

DNA fragment

Primer

Sequence of primer

rpb1

Hrpb1F (Forward)

5′-TGCAGTTCGTGCTCATTTTC-3′

Hrpb1R (Reverse)

5′-CGTGTCTACACGCTGTCGTT-3′

Hrpb1-1 (Forward)

5′-CAACAARCCTGTRATGGGWA-3′

Hrpb1-2 (Reverse)

5′-GCRGTRTCRATGAGACCYTC-3′

Hrpb1-3 (Forward)

5′-GCAGAGYGAGGAAACTCGAG-3′

Hrpb1-4 (Forward)

5′-AGCTTAGCCAAATCGCATGG-3′

Hrpb1-5 (Reverse)

5′-CGTTGRCCTTGTTCGATRTA-3′

tef1α

HEF1F (Forward)

5′-AATCTCTGGTTGGCATGGAG-3′

HEF1R (Reverse)

5′-TTCCATCGTCTTTCCTGTCC-3′

HEF1-1 (Forward)

5′-CATGTTGGAGGAATCWGTYAAG-3′

HEF1-2 (Reverse)

5′-GGGGTGRTTAAGAACGATRA-3′

HEF1-3 (Forward)

5′-GGCATGGAGACAACATGTTGGA-3′

HEF1–4 (Reverse)

5′-TCCGACGATCRATCTTCTCG-3′

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