Figure 3 | Scientific Reports

Figure 3

From: miRNA let-7b modulates macrophage polarization and enhances tumor-associated macrophages to promote angiogenesis and mobility in prostate cancer

Figure 3

Up-regulation of let-7b expression in prostatic TAMs.

(a) Let-7b expression in different macrophages. M0, M1, M0 and TAMs were differentiated as described in Materials and Methods. Let-7b expression was determined by real-time RT-PCR. Data represent mean ± SD of three independent RT-PCR results. The expression of miRNA was normalized to U6. (b) Expression of let-7b is down-regulated in TAMs treated by its inhibitor. TAMs were transfected with let-7b inhibitors, negative control (NC), or remained untreated for 72 h. Relative expression of let-7b was analyzed by real-time RT-PCR. The expression of miRNA was normalized to U6. Data represent mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.

Back to article page