Figure 2
From: Acute blockade of IL-25 in a colitis associated colon cancer model leads to increased tumor burden
Increased colitis and decreased eosinophils in CAC mice treated with IL-25 neutralizing antibody.
(A) H&E stained histology sections of colons from isotype (top) and α-IL-25 (bottom). Black boxes represent successive higher magnification from left to right with the black bar representing 100 μm. (B) Histological colon sections analyzed for colitis severity. (C) Single cell suspensions were made from colon tissue and labeled with fluorescently labeled antibodies to distinguish dendritic cells, eosinophils, macrophages, neutrophils, T and B cells and were analyzed by flow cytometry (percentage on left, absolute number on right). Results are presented as mean ± SEM and are representative from three independent experiments with 3–5 mice per group. *P < 0.05, **P < 0.005.
