Figure 5
From: Analysis of crystal structure of Arabidopsis MPK6 and generation of its mutants with higher activity
In planta kinase activity assays of the MPK6 mutants.
The MPK6 mutant proteins were immuno-precipitated (IP) from plants and subjected to an in-gel kinase assay with MBP as the substrate. Equal amounts of the immuno-precipitated (IP) MPK6 mutant proteins were used for the assays. Phosphorylation of MBP by the MPK6 mutants (top panel). Immunoblot (IB) analyses with the anti-MPK6 antibody indicated that equal amounts of the MPK6 mutants (bottom panel) were used in each reaction. (A) The MPK6 mutants were transiently expressed in tobacco leaves and immuno-precipitated (IP). The kinase activities of the MPK6 mutants were tested. (B) The MPK6 mutants were expressed in transgenic Arabidopsis plants and immuno-precipitated (IP). The kinase activities of the MPK6 mutants were tested.
