Figure 6
From: Analysis of crystal structure of Arabidopsis MPK6 and generation of its mutants with higher activity
Measurements of camalexin and ethylene production and observation of leaf senescence in the MPK6 mutants transgenic Arabidopsis plants.
The amount of camalexin (A) and ethylene (B) produced by the two-week-old MPK6 mutants transgenic seedlings were measured 12 hours after transgene induction. (C) Early leaf senescence of the four-week-old MPK6 mutant transgenic plants was observed 7 days after transgene induction. (D) MPK6 mutants kinase activities (top panel) and protein levels (bottom panel) in the transgenic Arabidopsis plants used for the camalexin and ethylene measurements and leaf senescence observations. The MPK6 mutant proteins were immuno-precipitated (IP) from 15 μg of total proteins and subjected to in-gel kinase assays and immunoblot analyses. In (A,B), the data represent the means ± standard deviations of three biological replicates for each treatment. Asterisks indicate statistically significant differences between the mutant seedlings and vector control seedlings. **P < 0.01 (paired t test).
