Figure 6 | Scientific Reports

Figure 6

From: Aberrant expression of the S1P regulating enzymes, SPHK1 and SGPL1, contributes to a migratory phenotype in OSCC mediated through S1PR2

Figure 6

FTY720 induces ERK and Akt deactivation.

Images show the inhibition of ERK (A) and Akt (B) phosphorylation in H357 and H400 cells following treatment with increasing concentrations of FTY720 (μM) in complete media for 24 hours. The intensity of the bands are normalized to α-tubulin and expressed relative to the cells treated with vehicle control (=1). Results are shown as mean ± SD values of three independent experiments. H357 and H400 cells were treated with either PI3K inhibitor (LY294002) (C) or MEK1/2 inhibitor (UO126) (D) in complete media prior to MTT assays. Results are shown as mean ± SD values of triplicates. *ρ < 0.05, **ρ < 0.01 and ***ρ < 0.001 (Dunnett’s post-hoc test).

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