Figure 2: Sequence alignment of the L. lactis BirA homologues with the E. coli BirA. | Scientific Reports

Figure 2: Sequence alignment of the L. lactis BirA homologues with the E. coli BirA.

From: Deciphering a unique biotin scavenging pathway with redundant genes in the probiotic bacterium Lactococcus lactis

Figure 2

The multiple alignment was performed using ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html), and the final output was processed using the program ESPript 2.2 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi). The well-studied BirA_ec protein was used from E. coli K-12 MG1655 (Accession no. AAC43075), and the two BirA homologues were derived from L. lactis IL1403. BirA1_LL (Accession no. NP_267937) is shown in red and BirA2_LL (Accession no. NP_268060) is indicated in blue. Identical residues are in white letters with red background, similar residues are in red letters with white background, varied residues are in black letters, and dots represent gaps. The predicted secondary structure of BirA is shown at the top of the alignment. α: α-helix; β: β-sheet; T: β-turns/coils. Designations: EC, E. coli; LL, L. lactis.

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