Figure 4

Dox downregulates BRCA1 in primary neurons.

(A) Cortical neurons at 28–32 DIV were treated with a vehicle or with Dox (0.1 μM) for 2 days, fixed and stained for BRCA1 (green), MAP2c (red) and the nuclear Hoechst dye (blue) and imaged. Note that, although BRCA1 is mostly nuclear, there is some perinuclear BRCA1 staining, which was observed in neurons before¹⁹. Scale bar is 5 μm. (B) Images of fixed neurons treated with Dox (0.1 μm) from (A) were analysed. ***p < 0.0001 (t test). (C) Cortical neurons were treated with a vehicle or with Dox (0.1 μM) for 2 days, fixed and stained for TBR1 (green), MAP2c (red) and with the nuclear Hoechst dye (blue) and imaged. Images were analysed for the nuclear TBR1 fluorescence intensities. N.S., non-significant. A.u., arbitrary units. One hundred neurons were analysed. Results were pooled from two independent experiments.