Figure 2 | Scientific Reports

Figure 2

From: IgA response and protection following nasal vaccination of chickens with Newcastle disease virus DNA vaccine nanoencapsulated with Ag@SiO2 hollow nanoparticles

Figure 2

(A) Zeta potential of Ag@SiO2 hollow nanoparticles, APTMS-Ag@SiO2 hollow nanoparticles and pFDNA-Ag@SiO2-NPs. Data are presented as the mean ± standard deviation (n = 5). (B) The combination of the plasmid pVAX I-F (o) DNA with Ag@SiO2 hollow nanoparticles, APTMS-Ag@SiO2 hollow nanoparticles. Lane 1 shows the plasmid DNA; Lane 2 shows the Ag@SiO2 hollow nanoparticles mixed with plasmid DNA; Lane 3 shows the APTMS-Ag@SiO2 hollow nanoparticles loaded the plasmid DNA (pFDNA-Ag@SiO2-NPs); M: DNA Marker DL 15000. (C) Detection of APTMS-Ag@SiO2 hollow nanoparticles loaded DNA about protection and delivery property. M: DNA marker DL 15000; Lane 1: the plasmid pVAX I-F (o) DNA; Lane 2: the plasmid pVAX I-F (o) DNA treated by DNase I for 30 min; Lanes 3-6: pFDNA-Ag@SiO2-NPs treated by DNase I for 30, 60, 120, and 180 min, respectively; and (D) Release behavior of pFDNA-Ag@SiO2-NPs in the PBS solution (pH = 7.4) at 37 °C. Data are presented as the mean ± standard deviation (n = 5).

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