Figure 1
From: Structural investigation into physiological DNA phosphorothioate modification
The properties of PT-dsDNA.
(A–C) The chemical structure of PT-free, Rp-PT and Sp -PT modification in DNA, respectively. The torsion angles were indicated. (D) The melting temperature of PT-free dsDNA (green), [Sp, Sp]-PT dsDNA (red) and [Rp, Rp]-PT dsDNA (blue) measured by DSC assay. (E) The electron transfer potentials of ssDNA measured by cyclic voltammetry assay of Sp-PT ssDNA (black), Rp-PT ssDNA (pink) and PT-free ssDNA (cyan). (F) The circular dichroism (CD) spectra of PT-free dsDNA (green), [Sp, Sp]-PT dsDNA (red), [Rp, Rp]-PT dsDNA (blue), respectively; (G–I) The electron transfer potentials of dsDNA containing buffer control, PT-free dsDNA (green), [Sp, Sp]-PT dsDNA (red), [Rp, Rp]-PT dsDNA (blue).
