Figure 3: Western blot and immunohistochemistry analyses for DAP validation. | Scientific Reports

Figure 3: Western blot and immunohistochemistry analyses for DAP validation.

From: Proteome-wide alterations on adipose tissue from obese patients as age-, diabetes- and gender-specific hallmarks

Figure 3

(A) IHC detection of TOM22 in VAT samples from 4 older and 4 younger non-diabetic patients showing over-expression of TOM22 staining (in brown) in younger adipose cells, mainly surrounding the nuclei where the mitochondrial are located (arrows). Magnification x200 under Nikon Eclipse 90i microscope. a, adipocyte. Scale bars, 50 μm. (B–D) Representative WB analyses of selected DAPs using an additional set of 32 VAT samples (8 per cohort) in age, T2DM and gender comparisons. The results were normalized for B-actin density. Underneath, the values for relative intensity obtained after densitometry of the bands are means ± SD. Statistical significance was set at p < 0.05.

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