Figure 4

The production of CCL24 is impaired in AMs from AQP3^−/− mice.

(a) Aqp3 mRNA expression levels in AMs. mRNA expression levels of Aqp3 in FACS-Aria sorted AMs (F4/80⁺CD11c⁺ cells) in the lungs (n = 4 for each control group; n = 6 for each OVA-challenged group). (b) Double staining of immunohistochemistry of AQP3 and F4/80 in the lung. Lungs of saline-challenged WT mice (left) and OVA-challenged WT mice (right) were dissected and stained with AQP3 antibody and F4/80 antibody. Arrowheads indicate AMs. Bars, 10 μm. (c) Concentrations of CCL24 in BALF on day 28 (n = 5 for each control group; n = 8 for each OVA-challenged group). (d) Ccl24 mRNA expression levels in BALF total cells (n = 4 on day 21; n = 7 on day 28) and AMs (n = 4 on day 21; n = 6 on day 28). **P < 0.01.