Figure 5: Functional genomics of the myelin transcriptome.

(A) Heat map showing distinct k-means clusters of transcripts in myelin of which the abundance differentially changes between any analyzed age. (B) Bar plot showing enrichment of biological processes for mRNAs in cluster 1 obtained from k-means clustering (compare Fig. S3G) representing abundant transcripts in myelin at age P18. Bars indicate numbers of mRNAs in the respective biological process (lower x-axis) while the line represents the respective p-value (upper x-axis). (C) Same as in Fig. 5B but for k-means clustered transcripts abundant in myelin at age P75. (D) Same as in Fig. 5B but for k-means clustered transcripts abundant in myelin at 6 and 24 months of age. (E) Same as in Fig. 5B but for k-means clustered transcripts abundant in myelin at 6 months of age. (F) Comparison of the mRNAs detected in myelin with the genes causing (when mutated) heritable disorders preferentially affecting the white matter of the brain (leukodystrophies/leukoencephalopathies). Note that surprisingly many leukodystrophy-associated mRNAs were identified in myelin at least at moderate or high abundance (see also supplemental Fig. S5).