Figure 2
(A) Fluorescence images (upper panels) showing increasingly larger autofluorescent aggregates attributed to lipofuscin vesicles in 3 neuronal preparations; 3 week old neurons cultured in the NBN2 medium (3rd columns) exhibit the largest aggregates. Each image size is 35 × 35 μm2. (B) Cholesterol concentration in the membrane pellets of hippocampal neurons at different stages of aging: 30% lower cholesterol levels were detected for 3-week-old neurons cultured in PNG compared to 1-week-old cells. Neurons cultured in NBN2 showed a more pronounced decrease in membrane cholesterol levels. *p = 0.1 for at least 9 independent data points per sample **p = 0.02 (unpaired Student t-test) for at least 7 data points per sample. Error bars represent the standard error of the mean for each sample.
