Table 1 Susceptibilities of different neuronal preparations to the binding of pre-aggregated Aβ40 and Aβ42 peptides and the elastic moduli recorded (set point = 0.2 nN).
Neuron age/type and Aβ treatment | Mean percentage of Aβ-positive (fluorescent) neurons | Coefficient of variation (CV) | Total number of neurons (bright field images) | Mean Young modulus (Pa) for shallow indentations (0.2 nN) | Std. error of the mean |
|---|---|---|---|---|---|
1w PNG control | – | – | – | 116.46 | 9.76 |
1w PNG + Aβ40 | 34% | 73% | 189 | 110.98 | 8.38 |
1w PNG + Aβ42 | 52% | 29% | 223 | 106.96 | 13.61 |
3w PNG control | – | 103.91 | 7.60 | ||
3w PNG + Aβ40 | 82% | 20% | 264 | 112.64 | 11.01 |
3w PNG + Aβ42 | 79.5% | 18.5% | 173 | 84.41*** | 9.44 |
3w PNG/NBN2 | – | 112.37 | 6.91 | ||
3w PNG/NBN2 + Aβ40 | 97.7% | 5% | 296 | 72.94* | 6.00 |
3w PNG/NBN2 + Aβ42 | 89.7% | 11% | 346 | 70.95** | 6.74 |
