Figure 2 | Scientific Reports

Figure 2

From: A single exercise bout enhances the manufacture of viral-specific T-cells from healthy donors: implications for allogeneic adoptive transfer immunotherapy

Figure 2

The effects of a single exercise bout on the frequency of viral-specific T-cells before (PRE) and after (POST) 8 days of viral-peptide stimulation.

The numbers of CMV pp65, CMV IE-1, EBV LMP-2 or EBV BMLF-1 specific T-cells were enumerated before and after expansion in a 12 h IFN-γ ELISPOT assay. Spot forming cells (SFC) were adjusted per 100,000 CD3+ T-cell (as opposed to total cells) to account for the greater proportion of NK-cells among the PBMC fractions after exercise. Panel A shows individual subject responses for all CMV/EBV seropositive participants (n = 9). The median values are indicated by the blue line and the red line is the results from a CMV/EBV seronegative participant (n = 1). Statistically significant difference between the before (PRE) and after (POST) exercise conditions indicated by (#p < 0.05). Representative ELISPOT results for a CMV/EBV seropositive and seronegative participant are shown in Panel B.

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