Figure 1: Comparison of Features of Semen Collected by Rectal Probe Electrical Stimulus Method (REM) and Penile Probe Electrical Stimulation Method (PEM).

(A) Comparison of motility, malformation and acrosome integrity of sperm in semen from REM and PEM, held at 37 °C for 30 min. (B) Comparison of motility of sperm in semen between REM and PEM, held at different temperatures for different durations. (C) Comparison of motility and malformation after exchanging sperm and seminal plasma between REM and PEM. (D) Comparison of motility and malformation after adding urea to seminal plasma. In (C,D), symbols “+” means existence and “−” is non-existence of semen components in each row. In addition, “+” for urea in (D) means urea added to a concentration similar to that in REM seminal plasma. Results are means ± s.e.m. Data are from four independent experiments. Statistical analysis was performed by one-way analysis of variance. *P < 0.05, **P < 0.01.