Figure 5: Metabolic profiling of LoVo cells treated with or without 10 μM ADMA for 96 h in serum- starved medium.
From: Effects of ADMA on gene expression and metabolism in serum-starved LoVo cells
(A–C) The PCA score plots for all three groups, C vs SS, and SS vs ADMA, respectively. (D) Summary of main differential metabolites and transcriptional changes of corresponding genes altered by serum starvation and ADMA in glucose metabolism and TCA cycle. The dashed arrows in metabolic processes represent reactions with multiple steps. The red and green Arabic numerals represent the transcriptional fold change of the genes of SS/C, as well as ADMA/SS, respectively. C: control group; SS: 96 h serum starvation group; ADMA: 10 μM ADMA for 96 h in serum starvation medium. HK: hexokinase; PFK: phosphofructokinase; PK: pyruvate kinase; PDH: pyruvate dehydrogenase; ACO: aconitase; IDH: isocitrate dehydrogenase; OGDH: α-ketoglutarate dehydrogenase; SDHA: succinate dehydrogenase; MDH: malate dehydrogenase; CS: citrate synthase; GOT: glutamate oxaloacetate transaminase; GLUD: glutamate dehydrogenase; GLS: glutaminase.
