Figure 5: Confirmation of LOD for ciprofloxacin resistance conferring genetic variants. | Scientific Reports

Figure 5: Confirmation of LOD for ciprofloxacin resistance conferring genetic variants.

From: Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes

Figure 5

Pooled MIPs were tested against DNA from strains defined above at an input of 1.5× the upper limit of the calculated LOD range. All samples were dual indexed and pooled before sequencing on the Illumina MiSeq platform. Variance analysis was performed on mapped sequencing reads to calculate coverage at the indicated genetic variant with coverage of all 16 replicates represented along with the mean and SD.

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