Figure 6: Performance of MIPs in a complex matrix.

Extracted whole blood samples spiked with WGA DNA from ciprofloxacin resistant (a) B. anthracis, (b) Y. pestis, and (c) F. tularensis were tested with pooled MIPs. All samples were dual indexed and pooled before sequencing on the Illumina MiSeq platform. Sequencing reads were mapped to reference genes and total mapped reads plotted versus the total number of genome copies is represented (The scale of the X and Y axes are Log 10). Error bars represent the standard deviation of three replicates. Cutoffs are shown for each gene as dashed lines.