Figure 7: MIP amplicon detection with HRM real-time PCR. | Scientific Reports

Figure 7: MIP amplicon detection with HRM real-time PCR.

From: Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes

Figure 7

High resolution melt primers for F. tularensis (a) gyrA C248T G259T and (b) parE ΔTTAAA were tested on amplicons generated from samples in Fig. 3. Data curves are represented as HRM melting peaks. HRM Cq comparisons of genomic DNA and amplicons resulting from the MIP protocol at the same input genomic DNA for (c) gyrA C248T G259T and (d) parE ΔTTAAA are shown (The scale of the X axes are Log 10). Each curve represents the average of 3 replicates. Error bars represent the standard deviation of three replicates.

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