Figure 8: Evaluation of cytotoxicity of B2088 to mammalian cells. | Scientific Reports

Figure 8: Evaluation of cytotoxicity of B2088 to mammalian cells.

From: Branched Peptide, B2088, Disrupts the Supramolecular Organization of Lipopolysaccharides and Sensitizes the Gram-negative Bacteria

Figure 8

(a) Dynamic monitoring of cell viability expressed as cell index (CI) by xCELLigence system. hTCepi (15,000 cells/well) cells were allowed to attach to the electrode surface and exposed to PBS or 2.5 mg/ml tobramycin (indicated by black arrow). (b) Effect of B2088 on cell index. The time of addition is indicated by black arrow. (c) Normalized CI values determined after the addition of B2088 at various time intervals. (d) Effect of cell density (30,000 cells/well) on cytotoxicity of B2088. (e) Cytotoxicity of cationic OM permeabilizers to hTCEpi cells at 125 μg/ml. The abbreviations used are: PLL – poly(L-lysine); PLO - poly(L-ornithine); PEI - linear polyethylenimine. For a comparison the sensogram for the cytotoxic surfactant Triton-X100 is also shown. For better clarity only average value is depicted in the figure without standard deviation. (f) Hemolytic activity of B2088 to rabbit erythrocytes.

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