Figure 2: γH2AX expression is increased in E13.5 Polμ−/− retinas.
From: Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina
(a) Active DNA repair was evaluated by Western blot in WT and Polμ−/− retinal protein extracts. γH2AX levels were compared with those of H2AX and H2AII. (b,c) γH2AX immunostaining (green) showed a focal distribution in WT and Polμ−/− retinas. (d) The density of γH2AX-positive cells was quantified in whole-mount retinas. (e) The number of γH2AX-positive foci per positive cell was also scored in dissociated retinal cells (n > 100). Histogram shows the mean ± standard error of mean (s.e.m.), as well as the individual values in (d). *P < 0.05 and ***P < 0.001 vs. corresponding controls.
