Figure 4: Neuronal death is selectively increased in E13.5 Polμ−/− retinas.
From: Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina
(a–f) Dissociated retinal cells from WT and Polμ−/− animals were immunostained for PCNA (red, in b) or TUJ-1 (red, in e), and processed for TUNEL (green, in b,e) Nuclei were counterstained with DAPI (blue, in a,d). Numbers of total proliferative cells (c) and neurons (f), as well as those of doubly labelled cells, were scored. (g,h) Whole-mount retinas were immunostained for Islet1/2 (red, in g) or Brn3a (red, in h) and processed for TUNEL (green, in g,h), and labelled cells were scored. Arrow heads indicate doubly labelled cells. Simply and doubly labelled cells were scored (i,j). Dissociated cells were also immunostained for Brn3a and processed for TUNEL, and scored (k). Histograms show values corresponding to individual mice, together with the mean ± s.e.m. *P < 0.05 and **P < 0.01 vs. corresponding controls.
