Figure 12: HPLC chromatogram showing muropeptide analysis of E. coli D456 PG which had been treated with Bd0468, Bd3279 or buffer (control).

New peaks can be detected (numbered 3–6) that are not present in the control. *peaks derived from purified Bd0468 protein solution. Peaks 1–6 were analysed by MS/MS (Table 2) which showed that Bd0468 and Bd3279 are peptidoglycan GlcNAc deacetylases.