Figure 3: Epifluorescent images of wild type B. bacteriovorus HD100 expressing and secreting C-terminally mCherry tagged Bd0468 or Bd3279 proteins whilst invading non-fluorescent E. coli at 45 minutes post-mixing. | Scientific Reports

Figure 3: Epifluorescent images of wild type B. bacteriovorus HD100 expressing and secreting C-terminally mCherry tagged Bd0468 or Bd3279 proteins whilst invading non-fluorescent E. coli at 45 minutes post-mixing.

From: Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts

Figure 3

Fluorescence was acquired with a two second exposure and maximum sensitivity gain. Despite the low intensity from the fluorescent tags on both proteins, they were clearly localised to the prey-bdelloplasts, with no tagged protein visible in the Bdellovibrio cells. Within the bdelloplasts, the outline of the invaded Bdellovibrio cell could be seen as a black backlit shadow against an mCherry background, indicating that the protein was exported into the bdelloplast periplasm and/or cytoplasm. Scale bars are 2 μm.

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