Figure 5: Epifluorescence phase contrast microscopy of Bdellovibrio (small, phase dark, comma-shaped cells) preying upon E. coli prey cells which have periplasms constitutively fluorescently labelled by a pMal::mCherry fusion (and cytoplasm labelled as the fusion protein is expressed). | Scientific Reports

Figure 5: Epifluorescence phase contrast microscopy of Bdellovibrio (small, phase dark, comma-shaped cells) preying upon E. coli prey cells which have periplasms constitutively fluorescently labelled by a pMal::mCherry fusion (and cytoplasm labelled as the fusion protein is expressed).

From: Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts

Figure 5

The images are from late in the predation cycle, with time post-mixing of predator and prey shown in minutes to the left of each image. With wild type B. bacteriovorus HD100 (A) and in 44% of the double mutant (B), total bdelloplast fluorescence is lost within 2 frames (<5 min; exit mode 1) as the inner membrane is breached by exiting predator. 56% of the double ΔBd0468Bd3279 mutants (C) display a different pattern, with fluorescence lost from the prey periplasm within 2 frames, but retaining fluorescence in the original prey cytoplasm compartment even after the Bdellovibrio cells (which in this case have grown between wall and outer membrane only), have become motile and broken out of the bdelloplast; (exit mode 2). Scale bars are 1 μm.

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