Figure 3
From: Simultaneous deactivation of FAK and Src improves the pathology of hypertrophic scar
The protein expression of p-FAK-Tyr407, FAK, p-Src-Tyr529, p-Src-Tyr416 and Src following TGF-β1 treatment in normal skin fibroblasts.
(A) Immunoblots show the expression of p-FAK-Tyr407, FAK, p-Src-Tyr529, p-Src-Tyr416 and Src in normal skin fibroblasts (NF) following TGF-β1 (10 ng/ml) or its vehicle control (VeCtrl, 10 mM citric acid) treatment at designated time points of 0, 1, 3, 6, 12 and 24 h. Actin serves as an equal loading control. (B,C) Bar graphs show changes on relative protein levels of p-FAK-Tyr407 (B), p-Src-Tyr416 and p-Src-Tyr529 (C) after TGF-β1 treatment over time. Each data point of the phosphorylated protein was normalized against its corresponding total protein level with the value at 0 h in TGF-β1 group arbitrarily set at 1. Each bar represents a mean ± SD of n = 4. *P < 0.05, **P < 0.01.
