Figure 1
In-vitro rhBMP-2 and ZA release kinetics.
(A) Indicates rhBMP-2 release (%) from the biphasic material over a period of 7-days detected using ELISA. (B) Shows the microscopic effect of free ZA (increasing concentrations using tissue culture plates (2D TCP)) and ZA released from the biphasic material (different day fractions) on A549 lung cancer cells (Yellow arrow indicates healthy, epithelial morphology of cells, red arrow points at round, dead cells while green arrow shows floating apoptotic bodies). (C,D) Fraction of ZA released (%) from the biphasic material over a period of 7-days and the cytotoxicity induced in A549 cells by the released fraction using the MTT assay, respectively. (E) Effect of bound and free ZA on A549 cells after seeding the cells directly on the biphasic material alone, in combination with ZA and plastic control treated with free ZA using the MTT assay. **Indicates p < 0.01, #indicates non-significant. Data is expressed as mean ± SD. Scale bar represents 30 μm.
