Figure 2

Transformed mitochondria identity confirmation and function within recipient cells.

(A) Confirmation of transformed mitochondria identity. Mitochondria were isolated from HeLa-DsRed2-mito cells and incubated (300 μg mitochondria/ml) for 1 h with HepG2 cells. Following the incubation the cells were washed 3 times with PBS and the cells were subjected to immunofluorescence staining with anti-NDUFS1 antibody and secondary 488-dye-conjugated antibody. Red-Dsred2. Green – 488 dye. Scale bar: 50 μm. (B) Mitochondrial transformation increases viability of recipient cells. Mitochondria were isolated from HeLa-DsRed2-mito cells and added to NDUFAF4-mutated fibroblasts and NDUFS2-mutated fibroblasts, at the indicated concentrations for 3 days. The cells were then washed 3 times with PBS and cell viability was tested. For control, mitochondria isolation buffer was used. Error bars represent ± SEM, n = 3, ^*P < 0.005. (C) Mitochondria were isolated from HeLa-DsRed2-mito cells, added to NDUFAF4-mutated fibroblasts and NDUFS2-mutated fibroblasts (450 μg/ml) for 3 days, followed by 3 washes with PBS. The activity of complexes II + III was measured. ^*P < 0.005. Error bars represent ± SEM, n = 3.