Figure 5: Inhibition of PRC1 causes alteration of gene regulation and polyploidy development during decidualization in vivo.
From: Polycomb repressive complex 1 controls uterine decidualization
Quantitative RT-PCR analyses of active genes (Alpl, Bmp2, Abp1, Cdkn1a, Trp53, Cdkn1c, Hoxa10, and Hoxa11) (A) and repressed genes (Tmem111, Rab28, Bcl3, SlC16a3, Nr5a1, and Gm7257) (B) during decidualization at the D8-IS after inhibition of PRC1 against control. (C) Flow cytometric analysis. Quantitative analyses of cell distribution (%) based on the DNA content are shown as insets for drug- or vehicle-treated groups. Quantitative analysis of polyploid cell count (%) without or with inhibition of PRC1 during decidualization. The error bars represent means ± SEM from three independent experiments. *Values are statistically different (P < 0.05). (D) Western-blot analysis reveals inhibition of PRC1, as judged by downregulation of H2AK119ub1, after addition of PRT4165 against control. Quantitation of H2AK119ub1 expression was achieved by direct analysis of the bands. Fold change was determined after normalization with H2A. *Values are statistically different (P < 0.05). These experiments were repeated at least three times with similar results.
