Figure 4 | Scientific Reports

Figure 4

From: Synergistic chemopreventive effects of curcumin and berberine on human breast cancer cells through induction of apoptosis and autophagic cell death

Figure 4

Apoptosis induced by co-treatment of CUR and BBR is mediated by activation of ERK.

(A) Cells were treated with 5 μM CUR and 25 μM BBR alone and in combination for 48 h. Protein extraction and Western blotting were then performed to determine the levels of ERK and p-ERK. MCF-7 (B) and MDA-MB-231 (C) cells were pretreated with U0126 (10 μM) for 1 h and then treated with CUR and BBR in combination for 48 h. (B,D) Cell viability was measured using MTT assay. (C) Protein extraction and Western blotting were performed to determine the levels of p-ERK, Bax and Bcl-2. GAPDH was used as the loading control. Values represent the mean ± SD (n = 3). **p < 0.01.

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