Figure 6 | Scientific Reports

Figure 6

From: Synergistic chemopreventive effects of curcumin and berberine on human breast cancer cells through induction of apoptosis and autophagic cell death

Figure 6

Autophagy contributes to the synergistic cytotoxicity of co-treatment of CUR and BBR.

(A) MCF-7 and MDA-MB-231 cells were pretreated with CQ (10 μM) for 1 h and then co-treated with CUR (5 μM) and BBR (25 μM) for 24 h. Protein levels of LC3-I, LC3-II, p62, Bax and Bcl-2 were analyzed by Western blotting. The cells were co-treated with CUR (5 μM) and BBR (25 μM) with or without CQ (10 μM) or 3-MA (10 μM) pretreatment. Cell death were then measured using LDH assay in MCF-7 (B) and MDA-MB-231 (C) cells. Values represent the mean ± SD (n = 3). **p < 0.01.

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