Figure 2: Evaluation of the TALE–DNA scaffold system by split GFP assay.
From: Spatial organization of heterologous metabolic system in vivo based on TALE
(a) Schematic of the manner of integration of split GFP to the TALE–DNA scaffold system and their hypothetical binding patterns to DNA BMs. The designed DNA BMs are shown at the top left of each panel. These patterns include the “head-to-head” pattern (upper panel) and the “BM1-interval-BM2-interval” pattern with 6 bp (middle panel) and 16 bp intervals (lower panel). (b) The green fluorescence (Ex: 488 nm; Em: 538 nm) of split GFP was examined after overnight culture of E. coli with or without IPTG induction. Relative fluorescence intensity was calculated with normalization of the OD600 value. The relative fluorescence intensity of the TALE1–GFP1/TALE3–GFP2 control group was set arbitrarily at 1.0, and the levels of the other groups were adjusted correspondingly. This experiment was run in three parallel reactions, and the data represent results obtained from at least three independent experiments. *p < 0.05, **p < 0.01. (c) Semi-qRT-PCR analysis of GFP1 and GFP2 expression in different TALE–GFP–scaffold groups. The cDNA sequence of 16S rRNA was amplified as the standard.
