Figure 4: The relative DNA quantification of representative sample at each time point.

The figure was generated by the normalized, individual data point 2−ΔCT43. The normalized, individual data point 2−ΔCT at the time point 0 min was used as benchmark, the results of other time points were compared with the benchmark. The multivariate analysis of variance process of the general linear model showed there was a significant difference between the two groups at the time points labeled with asterisks (P < 0.05). The representative sample A at the time points 2.5, 5, 10, 20, and 30 min, has corresponding P values of 0.005, 0.028, 0.001, 0.004, and 0.025 respectively.