Figure 5: 2-O-Bn-InsP₅ inhibits cell migration in different cell lines.

(A) Results from fibronectin-induced migration of MDA-MB-231 cells assessed by Transwell assays in the presence of 2-O-Bn-InsP₅ or InsP₅ (50 μM). Control cells were treated with vehicle alone. (B) MDA-MB-231 cells were pre-treated with the indicated concentrations of 2-O-Bn-InsP₅. Control cells were pre-treated with H₂O as control. Fibronectin-induced migration of MDA-MB-231 cells was assessed by Transwell assays. (C,D) Results from invasion of the indicated cancer cell lines assessed using transwell inserts pre-coated with Matrigel. Cells were treated with 2-O-Bn-InsP₅, InsP₅ (50 μM) or vehicle control. (E) Invasion of A375M transfected with siRNA targeting PLCγ1 (si-PLCγ1) or with a control non-targeting siRNA (si-control) untreated or treated with 2-O-Bn-InsP₅ 50 μM. In all panels data indicate the number of migrated or invaded cells/field and are expressed as percentage of control, vehicle-treated cells. Data are means ± SEM from n = 3 independent experiments. *p≤0.01; **p≤0.003; ***p≤0.001.