Figure 2

The effect of COS treatment on different mutants.
Mock means pretreated Arabidopsis with water and then inoculated with PBS by gently rubbing the leaf surface using Carborundum (silicon carbide). Mock + TMV or COS + TMV means pretreated Arabidopsis with water or 50 mg/L COS and then inoculated with TMV by gently rubbing the leaf surface using Carborundum (silicon carbide). (A) The necrotic lesion on leaves after inoculated with TMV for 7d in different mutants. (B) The amount of cell death in rosette leaves of Arabidopsis which inoculation with TMV 7d was stained with Evans blue. (C) The disease index which evaluated the necrotic lesion on leaves of different mutants. Values are the means ± SD from three independent measurements. Asterisks indicate significant differences (*P < 0.05, **P < 0.01; ***P < 0.001). (D) The amount of cell death in rosette leaves, by measured the absorption under 600 nm using spectrophotometer. Values are the means ± SD from three independent measurements. Asterisks indicate significant differences (**P < 0.01, ***P < 0.001).