Figure 3: Development of a miBDV vector system. | Scientific Reports

Figure 3: Development of a miBDV vector system.

From: Long-term expression of miRNA for RNA interference using a novel vector system based on a negative-strand RNA virus

Figure 3

(A) Schematic representation of a miBDV vector containing a cassette site for the insertion of miRNAs of interest. (B) Evaluation of BDV infection by IFA. (C) Northern blot analysis of rBDV-derived miRNAs. The expression of let-7 was used as a loading control. (D) Relative luciferase activity after transfection of pLuc-miR-Tubb3as or pLuc-miR-GAPDHas into Vero cells infected with rBDV-miR-Tubb3 or rBDV-miR-GAPDH. Full length blots of (C) are presented in Supplemental Fig. 4. Values are expressed as the mean + S.E. ****P < 0.001; n.s., no significance.

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