Figure 4: Knockdown of GAPDH and tubulin β3 expression in primary neurons.

Reduction of tubulin β3 (A) and GAPDH (B) expression in mouse hippocampal neurons infected with rBDV-miR-GAPDH and rBDV-miR-Tubb3, respectively. Mouse hippocampal neurons were infected with rBDVs on day 3 in vitro (DIV 3). On DIV 10, the mouse hippocampal neuron was stained with indicated antibodies. Arrowheads, the vector-induced neuritis; arrows, the vector-induced neurons. Bars, 10 μm.