Figure 2
From: Reduced tonic inhibition after stroke promotes motor performance and epileptic seizures
Stroke down-regulates extrasynaptically located GABAA receptors containing the δ subunit.
(A) qPCR analysis revealed a significant decrease of subunit δ, α4 and β3 transcript abundance. Transcript abundance of all other GABAA receptor subunits tested remained stable. Data were normalized to Tubb3 and displayed as the geomean of ratios ± s.e.m. [rats (n = 6), mice (n = 4), ipsi vs. contra, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001]. Left column: rats; right column: mice (B) Western blot analysis revealed a significant decrease of subunits δ, α4 and β3 and an unaltered expression of subunit α5 7 days after MCAO. Data were normalized to ß-III-Tubulin. Ratios of optical densities are shown as the percent relative to the contralateral hemisphere. Bars represent the mean ± s.e.m. [rats (n = 5), mice (n = 4), ipsi vs. contra, *p ≤ 0.05, **p ≤ 0.01]. (C) Representative protein bands are shown. Selected protein bands were cropped as indicated by dotted lines. All gels were run under the same experimental conditions. Ipsi- and contralateral brain samples from the same animal processed for one receptor subtype were always run on the same gel. Used antibodies and protein size of GABA receptor subunits (in kDa) are summarized in Supplementary table 1.
