Figure 4
From: Reduced tonic inhibition after stroke promotes motor performance and epileptic seizures
Diminished tonic GABAergic inhibition promotes post-stroke motor function.
(A) Mice showed improved RotaRod performance 3 and 7 days after stroke [controls (n = 14), MCAO (n = 8–10), #p ≤ 0.01]. Post-stroke activation of δ-GABAARs via gaboxadol (THIP) reduced motor function to control levels [MCAO (n = 8–10), MCAO + THIP (n = 8), *p ≤ 0.001]. THIP had no effect on motor performance in control mice (n = 5, Fig. 4A). Data points represent the mean ± s.e.m. [time on RotaRod vs. pre-surgery in percent]. (B) NMDA-receptor blockade by MK-801 reversed the improved RotaRod performance at 3 and 7 days following stroke. Bars represent the mean ± s.e.m. [control mice (n = 15), control + MK-801 (n = 14), MCAO (n = 8–10), MCAO + MK-801 (n = 12), *p ≤ 0.001, #p ≤ 0.05 at 3 days, #p ≤ 0.001 at 7 days, §p ≤ 0.001 at 3 days). (C) Motor function as assessed by RotaRod was better in GABRD+/− mice compared to their wild-type littermates (GABRD+/+). Bars represent the mean ± s.e.m. [time on RotaRod in sec, GABRD+/+, GABRD+/− (n = 11 each), *p ≤ 0.01].
