Figure 2
From: Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage
Complementation of ΔRNF4 and ΔRNF4 ΔUBE2W mutant defects.
(a) Wild type cells; ΔRNF4 and ΔRNF4 cells complemented with chicken mCherry-Rnf4 as described in Fig. 1 were subjected to colony survival assay after treatment with the indicated concentration of HU. (b) Wild type cells; ΔRNF4; ΔRNF4 ΔUBE2W and ΔRNF4 ΔUBE2W complemented with Human Ube2w isoform2 were subjected to colony survival assay with the indicated concentrations of HU. Data represented as indicated: Wild type (Blue losange), ΔRNF4 (red square), ΔRNF4 ΔUBE2W (green triangle), rescue clone (circle opal). Error bars represent 2 SD.
