Figure 5
From: Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage
Prolonged DNA damage induced foci formation in ΔRNF4 cells is suppressed by UBE2W inactivation.
After treatments with or without MMC (50ng/ml) for 1H, cells were allowed to recover for 4H or 16H. (a) For each indicated cell line, the formation of MMC induced foci for γ-H2ax (green) and Rad51 (red) was analysed by immunostaining. Chromatin was visualized by DAPI (Blue). Representative images are shown for each staining and merged images. White bar: 10 um. (b,c) percentage of cell containing no focus; 1–5 foci; >5 foci are represented for each conditions respectively in blue, red and yellow. (a) γ-H2ax. (b) Rad51 quantification. More than 100 cells have been counted for each condition.
