Figure 2: Sirt6-deficient BMMs impaired osteoclast differentiation.
From: Sirt6 cooperates with Blimp1 to positively regulate osteoclast differentiation
(a) BMMs from 6 week-old Sirt6fl/fl and Sirt6fl/flMx1Cre mice were cultured in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml) for 5 days and stained with TRAP. Number of TRAP+ MNCs (>5 nuclei) was counted as osteoclasts. Scale bar, 200 μm. *P < 0.01. Data are represented as mean ± S.D. (b) Quantitative real-time PCR analysis of Sirt6, Nfatc1, and Atp6v0d2 mRNAs in Sirt6fl/fl and Sirt6fl/flMx1Cre BMMs stimulated with RANKL. *P < 0.05, **P < 0.01. Data are represented as mean ± S.D. (c) As in (b), except that cell lysates were subjected to immunoblot analysis as indicated. GAPDH was used as a loading control.
