Figure 3: Expression analysis of the PGL1 gene cluster and the effect of PglR overexpression on transcription in Fg. | Scientific Reports

Figure 3: Expression analysis of the PGL1 gene cluster and the effect of PglR overexpression on transcription in Fg.

From: Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin

Figure 3

(A) Affymetrix gene expression: Change in gene expression as a function of the location on the genome with focus on the genes surrounding the PGL1 gene. The change in expression level is shown relatively to 0 H for the six analyzed time points (0, 24, 48, 72, 96 and 144 h). (B) Expression analysis (RT-PCR) of the putative PGL1 gene cluster in the wild type and pglR overexpression strain. The lower gel shows the positive control with genomic DNA from the wild type. The arrows under the gel show gene orientation in Fg. (C) Expression analysis (RT-PCR) of genes surrounding the PGL1 locus in the Fg wild type, ΔPGL1, and O-PGL1 strains, with wild type gDNA as a positive control.

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