Figure 4: Phenotypes of the generated Fg strains.

(A) Cultivated on solid DFM medium and perithecium formation on carrot agar. (B) Medium fraction from liquid cultures of wild type, ΔPGL1, EO-pglR and O-PGL1 strains cultivated for ten days in liquid DFM. The medium was filtered and centrifuged to remove cell debris and non-soluble metabolites.