Figure 4

Secretion of pro-inflammatory cytokines from BMDM incubated on controlled nanotopography surfaces.

BMDM cultured from wild-type, ASC^−/−, NLRP3^−/− or AIM2^−/− mice were incubated on controlled nanotopography surfaces before being activated with LPS and ATP for the detection of IL-1β (a) or activated with LPS for the detection of TNFα (b) and IL-6 (c). Cytokine levels were expressed as cytokine (ng/ml) per cell to account for differences in the numbers of adherent cells. Results are expressed as mean ± SEM and are representative of three independent experiments. The p values were calculated in reference to the glass control surfaces for each murine genotype using a one way analysis of variance with a Dunnett’s post-test. *p < 0.05.