Table 1 Triazole binding to affinity purified wild type and ScErg11p6 × His Y140F/H.

From: Triazole resistance mediated by mutations of a conserved active site tyrosine in fungal lanosterol 14α-demethylase

ScErg11p

Triazole

[Azole]0.5

Kd (μM)

Hill number

Wild-type

FLC

0.43

0.074 (±0.015)

3.0

VCZ

0.40

0.082 (±0.018)

2.7

ITC

0.28

0.123 (±0.027)

1.6

PCZ

0.32

0.078 (±0.023)

2.2

Y140F

FLC

0.33

0.13 (±0.05)

1.86

VCZ

0.27

0.03 (±0.02)

2.60

ITC

0.33

0.13 (±0.07)

1.83

PCZ

0.29

0.15 (±0.07)

1.57

Y140H

FLC

0.27

0.11 (±0.06)

1.68

VCZ

0.29

0.22 (±0.07)

1.23

ITC

0.25

0.13 (±0.06)

1.48

PCZ

0.23

0.10 (±0.04)

1.57

  1. Values in brackets indicate standard errors. IC50 value is denoted as [Azole]0.5.
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