Figure 3: Network-assisted identification of novel genes for virulence and drug resistance.

(A) The survival curve of C. elegans fed with E. coli OP50, wild-type and each of six PAO1 mutants. Average survival time in day of 90 worms for each strain is indicated in the parenthesis. Three mutants conferred a significantly increased survival rate (blue lines) whereas three other mutants significantly decreased the survival rate (red lines) compared to that of wild-type PAO1. The degree of difference was statistically significant in both cases as determined by a log-rank analysis (P < 0.05). (B) Disc diffusion antibiograms of wild-type PAO1 and four mutants with ceftazidime are shown. Filter discs with increasing concentrations of ceftazidime (as indicated at the top) were placed on LB agar plates and inoculated with the indicated bacterial strains. After overnight growth, the cleared zones of inhibition were visualised. The numbers below disc plates indicate diameters of inhibition zone (mm).